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Accurate quantification of multidrug resistance-1 gene ( MDR1 ) expression in target cells would be of important therapeutic value in predicting cellular response to anticancer drugs. Because certain normal cells in peripheral blood physiologically express  MDR1 , increasing the sensitivity of the detection methods might result in confounding low-degree expression in tumor cells with physiologic expression in normal cells. The purpose of this study was to determine  MDR1  mRNA expression levels in peripheral blood leukocytes obtained from healthy adult volunteers using a competitive nucleic acid sequence-based amplification (NASBA) assay. We determined the reference intervals of  MDR1  mRNA expression in peripheral blood obtained from 98 healthy adults by measuring its expression with the quantitative NASBA assay between 5.50 × 10 4  copies/µg RNA and 6.76 × 10 5  copies/µg RNA. The new reference intervals were evaluated using a number of sensitive or resistant cell lines as control; positive or negative  MDR1  expression was clearly demonstrated. We also reevaluated  MDR1  expression levels in leukemia cells obtained from patient peripheral blood; 18 of 31 samples (58%) exceeded the newly established upper reference limit. The cutoff value established could be used to distinguish significant  MDR1  expression in tumor cells from physiologic expression in certain normal cells coexistent in peripheral blood.

clinical chemistry and laboratory medicine (cclm)

Expression level of  MDR1  message in peripheral blood leukocytes from healthy adults: a competitive nucleic acid sequence-based amplification assay for its determination