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We studied the uncertainty of measurement for the calcium and glucose (amount of) substance concentrations in serum. The evaluation follows a four-step procedure, which complies with the ISO document Guide to the Expression of Uncertainty in Measurement (GUM). The applications were chosen to represent commonly used measuring systems in medical laboratories. The uncertainty components are quantified using observations of the measuring system, and information from calibration certificates, instrument specifications and literature. The evaluation focuses on the measurement step but empirical terms are used to illustrate how the pre-analytical phase and patient-related issues can be accounted for.

clinical chemistry and laboratory medicine

Evaluation of Uncertainty of Measurement in Routine Clinical Chemistry  - Applications to Determination of the Substance Concentration of Calcium and Glucose in Serum