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The concentration of nucleosomes is elevated in blood of patients with diseases which are associated with enhanced cell death. In order to detect these circulating nucleosomes, we used the Cell Death Detection-ELISA plus  (CDDE) from Roche Diagnostics (Mannheim, Germany) (details at http:\\biochem.roche.com). For its application in liquid materials we performed various modifications: we introduced a standard curve with nucleosome-rich material, which enabled direct quantification and improved comparability of the values within (CV intraassay :3.0–4.1%) and between several runs (CV interassay :8.6–13.5%), and tested the analytical specificity of the ELISA.

Nucleosomes in Serum as a Marker for Cell Death