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Aberrant DNA methylation has been identified as an important mechanism for inactivation of tumor suppressor genes and mismatch repair genes during carcinogenesis. We used b̲i̲sulfite treatment and the P̲CR s̲ingle strand conformation polymorphism (S̲SCP) (BiPS) technique to analyze methylation status of the promoter regions of the  hMLH1 ,  p16 , and  HIC1  genes in several cancer cell lines and colorectal cancer tissues.

Heterogeneity of DNA Methylation Status Analyzed by Bisulfite-PCR-SSCP and Correlation with Clinico-Pathological Characteristics in Colorectal Cancer