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17-Hydroxypregnenolone (3β, 17α-dihydroxypregn-5-en-20-one) and pregnenolone (3β-hydroxypregn-5-en-20-one) were determined by radioimmunoassay following HPLC separation in serum of healthy subjects of both sexes from 2 to 66 years old (29 girls, 85 women, 30 boys, 89 men). The effects of age and sex on the levels of both steroids were investigated and the upper limits of normal in age groups were determined.

Age Relationships and Sex Differences in Serum Levels of Pregnenolone and 17-Hydroxypregnenolone in Normal Subjects