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To identify pre-β-high density lipoproteins, a rapid  two-dimensional separation by electrophoresis (1 hour 30 minutes) was performed  on an automated Phast System™. This procedure used commercially available  polyacrylamide gradient gels (4–15 %) and allows sensitive and reproducible  results. Pre-β-1- and pre-β-2-high density lipoproteins were clearly identified  by this method. In addition, our procedure was successfully applied to diagnosis  of a patient with familial lecithin: cholesterol acyltransferase deficiency,  characterized by the absence of α-high density lipoproteins.

clinical chemistry and laboratory medicine

Rapid Electrophoretic Separation of Pre-ß-Migrating High Density Lipoproteins Using Automated PhastSystem(tm): Application to Analysis of Lecitihin: Cholesterol Acyltransferase-Deficient Plasma