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Urinary  trans,trans -muconic acid is a sensitive biomarker for low level benzene exposure. The method described by Ducos  et al . (Int Arch Occup Environ Health 1990; 62:529–34) is commonly used for its determination. In this study, we demonstrate that N-benzoyl,  L -glutamic acid added to urine samples is a suitable internal standard to control  trans,trans -muconic acid recovery after solid phase extraction of urine and to compensate for variations which might occur during high-performance liquid chromatography analysis.

N-Benzoyl, L -Glutamic Acid as a Suitable Internal Standard for the Analysis of trans , trans -Muconic Acid in Human Urine by Liquid Chromatography