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We compared a spectrophotometric screening test measuring faecal porphyrin concentration with an HPLC method. There was a good overall correlation between both methods although some scatter was observed. ROC plot analysis of the screening test leads to a cut-off value of 35 nmol porphyrin per g faeces, wet weight with a sensitivity of 97% and a specificity of 96%. These results indicate that the screening test is quite useful for detection of increased total faecal porphyrin concentration, but less useful in accurate measurement of increased total faecal porphyrin concentration.

Evaluation of a First-Line Spectrophotometric Screening Test for Increased Faecal Porphyrin Concentration