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An improved assay for quantification of urinary porphyrins by use of second-derivative spectroscopy is described. A new method for calculation of the porphyrin concentration is developed and the whole procedure is computerized. Acidified urine samples can be assayed within a few minutes by using this method. Precision and recoveries for both uro- and coproporphyrin are good. The method is presented as a very fast and accurate assay for the screening and quantification of urinary porphyrins.

An Improved Method for Quantitative Determination of Urinary Porphyrins by Use of Second-Derivative Spectroscopy