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Sensitive enzyme immunoassays for the determination of total leukocyte neutral proteinase inhibitor and polymorphonuclear elastase-leukocyte neutral proteinase inhibitor complexes are described. The usable ranges of the standard curves were from 80 ng/l to 10 micrograms/l. The relative intra-assay coefficients of variation of the tests were between 2 and 4%, and the inter-assay coefficients of variation between 4 and 10%. In vitro and in vivo studies were performed with septic pigs and isolated leukocytes. The results show that leukocyte neutral proteinase inhibitor can be used in pigs as a parameter for the development of a septicaemia.

clinical chemistry and laboratory medicine

Determination of Leukocyte Elastase-Inhibitor Complexes and Leukocyte Neutral Proteinase Inhibitor by Enzyme Immunoassays. Leukocyte Elastase-Inhibitor Complexes in Porcine Blood, III.