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The wavelength accuracy of ten different types of spectrophotometer was tested with holmium perchlorate solutions. It was found to be good, with mean deviations from the literature values of maximally 0.3 nm. Standard deviations over the entire spectral range were within 0.75 nm. The absorbance accuracy for different types of instruments was generally within 5%, except in the 287 nm range where higher deviations were found. The sharpness of the holmium peaks, in combination with band width and sensitivity of the instruments, troubled the majority of the participants. 150 spectrophotometers were involved in the surveys. Linearity of the spectrophotometers was tested with p-nitrophenol and cobaltous sulphate and found to be satisfactory.

clinical chemistry and laboratory medicine

Simultaneous Spectrophotometric Calibration of Wavelength and Absorbance in an Interlaboratory Survey Using Holmium Oxide (HO 2 O 3 ) in Perchloric Acid as Reference, Compared with p-Nitrophenol and Cobaltous Sulphate Solutions (1978-1984)