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The antisera used were raised in rabbits against a 19-nortestosterone-7 alpha-carboxyethylthioether conjugate of bovine serum albumin. Tritium-labeled nortestosterone was used as tracer. Cross reactivities with metabolites of nandrolone, other anabolics and endogenous steroids were very low. To achieve additional specificity a clean up and separation procedure was developed, using isocratic high performance liquid chromatography. So far no qualitative discrepancies have been observed between the assay described here and confirmative determinations by combined high performance liquid chromatography-gas chromatography-mass spectrometry.

A Speciflc Radioimmunoassay for the Detection of 19-Nortestosterone in Urine