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: A method is described for the rapid determination of flecainid (Tambocor) in human plasma. Flecainid is removed from alkalized plasma by binding to a C-18 extraction column. After washing the column with methanol/water (1 + 1, by vol.) and acetonitrile/water (45 + 55, by vol.), the drug is extracted with methanol and determined by HPLC. Sample preparation takes about 15 minutes. With this method, flecainid can be determined directly in the concentration range 100-2000 micrograms/l, with an accuracy of 5-10% depending on the concentration. The results agree well with those obtained by the longer established but lengthy liquid extraction method.

clinical chemistry and laboratory medicine

Bestimmung der Konzentration eines neuen Antiarrhythmicums, Flecainid, im Plasma durch Hochleistungstlüssigkeitschromatographie (HPLC): Probenvorbereitung durch Extraktionssäulen