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The binding of [125I]CEA to an anti-CEA Sepharose-4B immunosorbent has been investigated in the presence of detergents. Non-ionic detergents (Triton-X-100, Lubrol PX and Tørgitol NPX) at concentrations of 5-40 milligrams did not inhibit the binding of [125I]CEA to the immunosorbent. Sodium deoxycholate decreased the binding from 98% (concentration of detergent 0.6 milligram) to 41% (concentration of detergent 20 milligrams). Sodium dodecyl sulfate inhibited the binding of [125I]CEA to the immunosorbent considerably, even at low concentrations.

Binding of Carcinoembryonic Antigen (CEA) to an Anti-CEA Immunosorbent in the Presence of Detergents