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Summary: A radioimmunoassay (RIA) without chromatography for the determination of 5a-androstan- 3a,17j3-diol and of 5a-androstane-3(3,17/3-diol in human plasma by using highly specific antisera against the 15/3-carboxyethylmercapto-bovine serum albumin conjugates is described. It could be shown that the 13% cross reaction of the 5a-androstane-3/3,17/3-diol antiserum with pure androst-5-ene-3/3,17/3-diol was negligibly low in plasma extracts. Sensivity, accuracy, precision, and linearity revealed results which comply with the requisites of a reliable RIA. The plasma levels obtained were as follows (mean ± SD): 5a-androstan-3a, 17/3-diol: Normal males (N = 27) 0.86 ± 0.22 nmol/1; normal females (N = 10) 0.31 ± 0.07 nmol/1; hirsute females (N = 25) 0.60 ± 0.24 nmol/1; 5a-androstane3/3,170-diol: Normal males 1.47 ± 0.43 nmol/1; normal females 0.49 ±0.10 nmol/1; hirsute females 0.91 ± 0.32 nmol/1.

Unconjugated 5α-Androstan-3α,17ß-diol and 5α-Androstane-3ß,17ß-diol in Human Plasma as Measured by Radioimmunoassay without Chromatography