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Summary: A new method, based on the principle of competitive protein binding, is described for the determination of progesterone in urine. The sensitivity of the assay for progesterone is 0.24 jug/1 urine. The within-assay precision, as determined by the coefficient of variation, varies between 4 and 10%, the between assay precision between 9.2 and 17%. The recovery of progesterone is 104.3%. The average urinary progesterone excretion in normal men is 0.12 ± 0.10Mg/g creatinine. In the follicular phase of 7 normal women the urinary progesterone excretion is 1.4 ± 1.1 jLtg/g creatinine, in the luteal phase 7.7 ± 4.6 Mg/g creatinine. In normal pregnancy the urinary progesterone excretion rises significantly (y = - 11.1 ± l.lx, r = 0.66, n = 175, p < 0.001) from 1.5 to 25.7 Mg/g creatinine.

Die Bestimmung von Progesteron im Harn mit der kompetitiven Proteinbindungsmethode