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A time-saving protein binding assay for the simultaneous determination of cGMP and cAMP has been adapted for human urine, using [3H]cGMP, [14C]cAMP, protein fractions from calf skeletal and lobster tail muscles and the phosphodiesterase inhibitor SQ 20.009. Recovery, accuracy, and precision are approximately at the 10% limit. Good specificity and no interference were observed with diluted urine samples (10 to 20 times).

clinical chemistry and laboratory medicine

Time Saving Protein Binding Assay for the Simultaneous Determination of Guanosine 3′:5′-monophosphate (cGMP) and Adenosine 3′:5′-monophosphate (cAMP) in Human Urine