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The oxidation of /?-D-glucose by glucose oxidase to <5^glucono-lactone which spontaneously hydrolyzes to D-gluconic acid K s been widely and successfully used for the determination of glucose in clinical chemistry. The method is specific for -glucose (1) and has replaced procedures employing unspecific redox systems. In the presence of peroxidase the H2O2 formed in the glucose oxidase reaction oxidizes a chromogen in amounts equimolar to glucose. The color is measured photometrically. Several improvements to this method have been described. The widely used chromogen 0-dianisidine has been replaced by noncarcinogenic substances such as ethylbenzthiazolinsulfonic acid (ABTS) (2), diethylphenylenediamine (DEPPD) (3) and the use of iodine in the presence of metallic ions has been suggested. Peroxidase-has been replaced in some procedures by catalytic systems such as iodine-vanadate (5), ammoniummolybdate-iodine (3) or molybdic acid-iodine (4). K HLER and BRAUNFELD in a critical review (6) gave preference to the use of peroxidase over nonenzymatic redox systems. We have developed and used in our laboratory for two years a glucose oxidase method for the determination of glucose in which peroxidase is replaced by the redox system of iodine plus Na-molybdate. Only 100 μΐ of blood or ΙΟμΙ of serum or plasma are needed. The procedure requires only one pipetting step. The blank is zero. The reaction takes place in a weakly acid medium and is stopped by adjusting the pH to 12.7.

Manual and Automated Determination of Glucose in Blood with Glucose Oxidase and Molybdate/Iodide as Redox Catalyst