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A simple and well-tried test for serum albumin is described. It is based on the formation of a dye-combination between albumin and bromcresolgreen. The technique, which is of extreme simplicity, can be carried out in very little time and requires only a few microlitres of serum or plasma. The preparation of the reagents is easy, their storage very satisfactory and they are not expensive. The results obtained fit in well with the salt fractionation techniques with sodium sulfite and electrophoresis with cellulose acetate. It is very accurate; owing to its great sensitivity, the results can be read even on very simple filter photometers. The data indicate that the proposed technique will prove very useful, especially in daily hospital routine.

Eine vereinfachte Mikromethode zur Bestimmung von Serumalbumin mit Hilfe von Bromkresolgrün