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Experiments were carried out in which ovariectomized mice were injected with increasing doses of /2-sitosterol followed by quantitative determination of calcium, magnesium and phosphorus in the blood: 1. ß-sitosterol in large doses (25, 50, 75 and 100 %) significantly increased the calcium levels. Doses of 5 and 10 7*g had no effect. 2. Similar doses of the phytoestrogen induced a striking decrease in the magnesium concentrations which finally attained about half the control values. 3. Significant decreases in the level of phosphorus were evident after all dosage levels of /?-sitosterol, except 100 ytig, which caused only an insignificant reduction in the concentration of phosphorus.

Mineral Changes in the Blood of Ovariectomized Mice after Treatment with β-Sitosterol