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A new Separation method for the radioimmunoassay ofproteobormones: en^ymic proteolysis of the hormone with pronase A new method is reported for the separation of free and antibody-bound hormone. The separation is based on the enzymic proteolysis of the free proteohormone, while the antibody-bound hormone is not attacked. The radioimmunoassay of insulin is described as an example of the method, which permits the use of hormones of relatively low specific activity, labelled with soft emitter 125I. This is ad

Eine neue Trennmethode für Radioimmunoassays von Proteohormonen: enzymatische Proteolyse des freien Hormons durch Pronase