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The quantitative analysis of porphyrins is carried out by means of spectrophotom etry or fluorometry. Spectrophotometric determination offers an advantage in that extinction coefficients for the absorption maxima can be established under standard conditions which can be duplicated, in other laboratories; thus, comparable measurements are possible. The molar absorptivity and the stability of the porphyrins increase when a metal ion is incorporated into central position of the tetrapyrrole ring, forming metal complexes of the chelate type. Studies on copper, zinc, magnesium, nickel, and cadmium showed the absorption in the Soret band to be greatest for the zinc chelates. On the basis of this observation a sensitive Spectrophotometric method has been developed for the analysis of porphyrins. As a prerequisite the porphyrins must be in the form of their methyl esters.

Formation of Zinc Chelates from Porphyrin Methyl Esters for Spectrophotometric Analysis