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The heparin clearing test was performed on 12 patients with /?-, y-plasmocytoma paraproteins respectively, and on one patient with Macroglobulinaemia Waldenstroem while 7 healthy persons served as controls. Serum turbidity, total fat concentrations, one-dimensional electrophoresis of lipids and proteins were determined in the sera before and after i. v. application of 12500 I. U. of heparin. After the administration of heparin, all plasmocytoma and Macroglobulinaemia paraprotein, as well as the control sera, showed the serum turbidity clearing effect, the decrease of total fats and the acceleration of the electrophoretic mobilities of the exand slipoproteins. Contrary to this, the plasmocytoma s-, yand Macroglobulin-paraprotein lipids showed specific behaviour in the post-heparin sera: they were resistant to the heparin acceleration effect and migrated before and after heparin administration with the same electrophoretic mobility as the corresponding paraproteins. This permits a clear separation of the serum paraprotein lipid component from the j5-lipoproteins in post-heparin sera and can be of use in the diagnosis of and y-plasmocytomas and of Macroglobulinaemia Waldenstroem respectively.

The Specific Property of Paraprotein Lipids in Serum Concerning the Heparin Clearing Effect