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After a preliminary hydrolysis of the serum samples, the liberated sialic acid is determined by the thiobarbituric acid method of WARREN adapted to the Autoanalyzer. By measuring the developed chromogen in very hot solution, the use of extraction or the addition of stabilizing agents is avoided. Sixty determinations can be run per hour and the optical densities follow a straight line well within the physiological range of concentrations.

The Automatic Determination of Blood Serum Sialic Acid Levels