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BRAF and NRAS mutation detection is crucial for advanced melanoma treatment. Our aim was to evaluate how different characteristics from formalin-fixed paraffin-embedded (FFPE) samples, age of the block or DNA concentration could influence the success of BRAF and NRAS mutational screening.

clinical chemistry and laboratory medicine (cclm)

Time and tumor type (primary or metastatic) do not influence the detection of  BRAF/NRAS  mutations in formalin fixed paraffin embedded samples from melanomas