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Background:  Reference change values (RCV) are used for judging the significance of changes between any two measurements. Based on the within-individual CV ( CV   I  ), RCV is conventionally computed as the 95% confidence limit (CL) of the changes:R  C  V  =  1.96  ×   2   C   V  I   .    $RCV = 1.96 \times \sqrt 2 C{V_I}.$    However, the appropriateness of assuming a constant  CV   I   and using the 95% CL for RCV remains controversial.

clinical chemistry and laboratory medicine (cclm)

Derivation of level-specific reference change values (RCV) from a health screening database and optimization of their thresholds based on clinical utility