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Background:  Previous studies have shown that the measurement of vitamin D and its derivatives, especially its active metabolite 1α, 25-dihydroxy-vitamin D [1,25(OH) 2 D], is highly complex and prone to analytical error. We have evaluated a new immunological method for detecting and quantifying of 1,25(OH) 2 D. This assay is fully automated, sensitive and uses a specific recombinant fusion protein for capturing of 1,25(OH) 2 D. The assay was originally developed by DiaSorin for the immunoassay analyzer LIAISON XL.

Experience with the first fully automated chemiluminescence immunoassay for the quantification of 1α, 25-dihydroxy-vitamin D