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Background:  Calprotectin is a 36 kDa calcium and zinc binding protein. An increased level of calprotectin points towards inflammatory bowel disease. However, the biomarker calprotectin shows 14 potential cleavages sites for trypsin. Next to trypsin, also the presence of its inhibitor α 1 -antitrypsin after a gastrointestinal bleeding may affect calprotectin testing. In this study, effects of trypsin and α 1 -antitrypsin as potential confounders for faecal calprotectin testing are investigated.

Proteolysis is a confounding factor in the interpretation of faecal calprotectin