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Background:  Lactase persistence is an inherited autosomal dominant trait that confers the ability to digest lactose after weaning. Lactose persistence is caused by single nucleotide variants in a regulatory element for the lactase gene ( LCT ). In Europeans, lactase persistence is associated with the nucleotide variant  LCT  -13910C&gt;T. Ethnic groups from Africa and the Arabian Peninsula have other lactase persistence variants in close proximity to the -13910C&gt;T variant. Current hybridisation probe analysis methods have been limited by the inability to detect all the known nucleotide variants. The aim of this study was to devise a method based on hybridisation probes and melting curve analysis for rapidly detecting  LCT  variants.

Lactase persistence genotyping: rapid detection of seven sequence variants in a single tube with melting curve analyses