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Differentially expressed genes in  Dunaliella salina  grown under super saturated salt stress (5.5  m ) were isolated by subtractive hybridization, cloned and classified for further biotechnological and industrial exploration. Sequence homology analysis showed that 26% of the library represented salt responsive genes for metabolic enzymes, viz., fructose-1,6-diphosphate aldolase ( AldP ), glucose-6-phosphate dehydrogenase, carbonic anhydrase, phosphoglycerate kinase etc., while 6% of library clones contained hypothetical/unknown genes. Six clones (8% of the library) contained genes that differentially expressed under salt stress, however, 6–8% of the library represented genes over expressed under salt stress and involved in photosynthesis, defense, cell rescue (chaperon/protease inhibitor) and protein sorting. About 39% were other genes of which 6% and 14% were transcription factors and house keeping genes (putative enzymes/signaling) respectively, while the remaining 19% of genes had miscellaneous functions.

Cloning differentially expressed salt induced cDNAs from Dunaliella salina under super saturated salt stress using subtractive hybridization