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Uptake rates of dissolved organic nitrogen (DON; urea, alanine and glutamate) by  Ulva pertusa  were determined using axenic cultures and  15 N isotopic tracers. When urea or alanine was added to N-starved cultures, the incorporation of  15 N into algal tissue was found at all concentrations tested (0.5–40 μM). Maximum uptake rates were 7.92 and 3.00 μmol N g −1  dw h −1 , and half-saturation constants were 25.5 and 27.6 μM for urea and alanine, respectively. These results show that  U. pertusa  can take up urea and alanine directly without bacterial decomposition to inorganic nitrogen. Therefore,  U. pertusa  is able to utilize certain species of DON, and has the potential to regulate DON fluxes and transformations in coastal environments.

Short-term uptake of dissolved organic nitrogen by an axenic strain of Ulva pertusa (Chlorophyceae) using [15]N isotope measurements