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Random Amplified Polymorphic DNA (RAPD) markers were developed for genotyping in  Laminaria digitata . Reproducible RAPD banding patterns were reliably obtained, using a quick micro-scale DNA extraction procedure. Intraspecific polymorphism was observed, allowing for unambiguous genotyping within sets of 3–4 sporophytes. A controlled crossing indicated that in this kelp RAPD markers are transmitted to the progeny in a Mendelian way. This approach was then applied to parentage analysis within a set of 30 anonymous sporophytes from a random cross experiment involving 4 parents. The parents of 26 offsprings were unambiguously identified, indicating that cross-fertilisation largely, if not totally, prevailed over self-fertilisation.

Development of RAPD Markers for Parentage Analysis in Laminaria digitata