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Human erythrocyte deformability is determined by cell geometry and volume, membrane elasticity and cytoplasmic viscosity. The deformability of red blood cells and their distribution among the total cell population, can be studied with the Capillary Rigidometer. This device is based on the kinetic measurement of red blood cell deformability, which has been developed, by modifying the micropipette aspiration technique. In order to investigate the validity of the method and the measuring parameters, a number of determining factors (heat treatment, osmolarity-changed cells) influencing the deformability were studied, and the sensitivity of the defined parameters for changes in deformability discussed. The results are examined in connection with different flow rates in the micropipette and show that the parameters are influenced by the flow conditions, so that they have to be related to these conditions. Initial studies using microspheres aimed at standardising the method are described.

biomedizinische technik

Bewertung der Ganzzelldeformierbarkeit einzelner Erythrozyten mittels Kapillar-Rigidometer - Möglichkeiten zur Standardisierung und Relativierung. Assessment of Erythrocyte Deformability with the Aid of the Capillary Rigidometer - Possibilities of Standardisation and Modifikation