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The examination of functional processes in tissue is gaining importance in medical research. As a result the imaging and monitoring of biochemical parameters  in vivo  is the goal of many imaging methods. One key parameter in photodynamic therapy (PDT) is the molecular oxygen concentration. Two-dimensional monitoring of oxygen is demanded for PDT but has not yet been achieved. The use of optical methods provides a possible means of measuring molecular oxygen. The basis of this method is the measurement of the luminescence lifetime of a dye that is quenched by molecular oxygen. The molecular oxygen concentration can be monitored two-dimensionally by pixel-wise determination of the luminescence lifetime with a CCD-camera. A new O 2 -imaging system based on this principle is presented in this article. The dye Ru(bpy) 3  2+  is quenched by molecular oxygen and was used in the first experiments with the system.

biomedizinische technik

An oxygen imaging system for medical applications: preliminary results / Ein Sauerstoffbildgebungssystem für medizinische Anwendungen: erste Ergebnisse