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Accurately measuring flow rates is a key requirement in many medical applications such as infusion and drug delivery systems. A major drawback of current systems is the low resolution of the sensors in the low flow rate regime. In this article, we present a method based on Holographic PIV/PTV that has been used for the first time to measure flow rates in the range of a few nL/min. Our method requires a very simple setup that combines lensless holography with particle velocimetry. For flow rates in the 70 to 200 nL/min range, the highest uncertainty was 5.6% (coverage factor  k =2). This is an open-source project; the CAD designs and software source code are available at  https://github.com/gui-miotto/holovel .

Holographic PIV/PTV for nano flow rates–A study in the 70 to 200 nL/min range