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Economical as well as medicinal and nutritional benefits of Japanese quails are important. The aim of the present study was to investigate the effect of dietary inclusion of probiotics and humic acids on some antioxidant, carcass and egg production parameters of Japanese quails. As Japanese quails are an interesting animal model attracting attention in the last years, a partial aim of this study is also a brief economical annex to the topic. A total of 60 birds (30 males and 30 females, age of 35 days) were involved in the experiment. Animals were fed by standard basal diet and divided to 6 experimental groups as follows: two groups (10 females and 10 males) received addition of probiotics preparation in a single dose of 1 g kg -1  of feed mixture, two groups (10 females and 10 males) received a preparation of humic acids in a single dose of 3 g kg -1  of feed mixture. The groups (10 females and 10 males) fed on basal diet without any additive served as the control group. After 210 days the animals were euthanized and blood samples, carcass organs and abdominal fat were collected. Antioxidant/oxidant parameters (activity of superoxide dismutase SOD, glutathione peroxidase GPx, total antioxidant status TAS, content of bilirubin and reactive oxygen species ROS) from blood samples were measured. The production of eggs was recorded during the experiment. Probiotic and humic acids treatments resulted in significant increase of TAS in females in contrast to males. Content of bilirubin was significantly lower in females fed with addition of humic acids in comparison to the male group. In both treatments (probiotics and humic acids) significantly lower amount of abdominal fat in females in comparison to the controls were recorded. The other investigated parameters of Japanese quails were not influenced by the treatments. The effect of probiotics and humic acids is probably dose dependent and the assessment of an accurate dose of substantives used in poultry feeding plays a crucial role.

Effectiveness of non-antibiotic stimulators in Japanese quail diet: Gender comparison and economical annex