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Aphis craccivora  and  Aphis fabae  were found to infest broad bean in Egypt.  Aphis craccivora  was found to be more dominant than  A. fabae; A. craccivora  was collected from nine locations that represented different agroecosystems in Egypt. At the same time,  A. fabae  was found in one location only. Single Nucleotide Polymorphisms (SNPs) in mtDNA Cytochrome c oxidase subunit I (COI) were used to differentiate  A. fabae  and geographically related populations of  A. craccivora  in Egypt. For this purpose COI primers were designed and registered in GenBank. The primers were successful in detecting genetic variations between both related  Aphis  species as well as among different geographic populations of  A. craccivora . Fourteen SNPs were found to differ among two biotypes of  A. craccivora  in the Nile Delta and Upper Egypt, probably due to environmental variations in the two different ecosystems.

Genetic differentiating Aphis fabae and Aphis craccivora (Hemiptera: Sternorranycha: Aphididae) populations in Egypt using mitochondrial COI