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In this paper, konjac oligoglucomannan (KOGM) was obtained with a hydrolysis rate of 56.24% by controlling the hydrolysis conditions. KOGM was passed through a 0.2 kDa dialysis bag, a 3 kDa ultrafiltration tube, and a 5 kDa ultrafiltration tube, creating samples with molecular weights of 0.2–3 kDa (IV), 3–5 kDa (III), and &gt;5 kDa (II), respectively. The  in vitro  antioxidant activities of the KOGM samples were tested by measuring their removal effects on ˙OH,O    2    −{\text{O}}_{2}^{-}   , and DPPH˙. The  in vivo  antioxidant activities of the samples were analyzed by measuring their impacts on the malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-PX) activity in mice. The results show that the KOGM samples in groups III and IV could effectively remove ˙OH,O    2    −{\text{O}}_{2}^{-}   , and DPPH˙; the KOGM samples in all three groups could enhance the SOD and GSH-PX activities and reduce the MDA content in the liver tissues of mice; finally, the antioxidant activity of KOGM is negatively correlated with the molecular weight.

Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities