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The identification and localization of N pi-methylhistidine (1-methylhistidine) within the primary structure (His 157) of rabbit skeletal muscle myosin-light-chain kinase is described. The data show for the first time that N pi-methylhistidine, known as a rare mammalian urine amino acid, is a protein constituent.

N π -Methylhistidine in Myosin-Light-Chain Kinase