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The sterically hindered tert-butyl thiol reacts smoothly with azodicarboxylic acid derivatives only upon addition of catalytic amounts of sodium alcoholate, yielding crystalline and analytically well characterized 1-(tert-butylthio)-1,2-hydrazinedicarboxylic acid derivatives. These sulfur-activated reagents were found to be stable on storage and well suited as tert-butylthio carriers for the introduction of this thiol-protecting group on cysteine, cysteine derivatives and cysteine peptides; the related compounds are obtained in high yields.

hoppe-seyler's zeitschrift für physiologische chemie

1-( tert -Butylthio)-1,2-hydrazinedicarboxylic Acid Derivatives. New Reagents for the Introduction of the  S-tert -Butylthio Group into Cysteine and Cysteine Derivatives