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A new hemoglobin is described. The hemoglobins of pig embryonic erythrocytes are separated by DEAE-Cephacel chromatography. In addition to a small amount of adult hemoglobin, four embryonic hemoglobins are obtained. Each of the hemoglobin components is analysed by globin and hemoglobin electrophoresis on polyacrylamide gels. Two of these hemoglobins are previously described as Hb Gower II (alpha 2 epsilon 2) and Heide I (zeta 2 epsilon 2). A tetrameric hemoglobin consisting of alpha and epsilon chains has been detected. Therefore, this new hemoglobin is named, in analogy, as Hb Heide II (alpha 2 epsilon 2). Furthermore, the other hemoglobin tetramer has been unequivocally identified as zeta 2 epsilon 2. This structure corresponds to Gower I which is only suggested in the human embryo.

hoppe-seyler's zeitschrift für physiologische chemie

Hemoglobins, XLI. The Embryonic Hemoglobins of Mammal. A New Hemoglobin, Hemoglobin Heide II (Hb He  II  : α 2 ϑ  2  ), and Demonstration of the Hemoglobin Structure of Gower I (ζ  2  ε  2  ) in the Pig Embryo