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The major component of membranes of microbodies from green leaves of Lens culinaris is a protein of a subunit molecular weight of 63 000. This protein, referred to as SP-63, seems to be unique to microbodies and could not be detected when plastids or mitochondria were analyzed. It is probably a structural protein and is thus not solubilized by cholate, Triton X-100, chloroform/methanol, or 0.2M KCl. Solubilization from purified membranes was achieved with guanidinium chloride or sodium dodecylsulphate. The protein was separated from minor contaminating components by chromatography on Sepharose 4B or Sephadex G-150 employing 0.1% sodium dodecylsulphate or 4M urea as eluent. It was shown to be homogeneous upon sodium dodecylsulphate gel electrophoresis and did not give a positive glycoprotein stain.

Plant Microbody Proteins, II. Purification and Characterization of the Major Protein Component (SP-63) of Peroxisome Membranes