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Cell-free extracts from Clostridium tetanomorphum, a microorganism which synthesizes corrins but no heme, are capable of converting both 5-aminolevulinate and uroporphyrinogen III into cobyrinic acid. Comparative examinations with (14C)5-aminolevulinate and (14C)uroporphyrinogen yielded corresponding results. Cell-free extracts from Clostridium tetanomorphum contain uroporphyrinogen III. To obtain good radiochemical yields it is therefore necessary to use substrates of high specific radioactivity. A method for the preparation of 14C-labelled uroporphyrin I-IV with high specific radioactivity is described.

hoppe-seyler's zeitschrift für physiologische chemie

Bildung von Cobyrinsäure mittels eines zellfreien Systems aus Clostridium tetanomorphum. Vergleichende Untersuchungen mit [ 14 C]5-Aminolävulinat und [ 14 C]Uroporphyrinogen