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Reaction of methyl vinyl ketone with bovine insulin affords, amongst others, a crystalline A1-(3-oxobutyl)insulin. This retains full biological activity in the mouse convulsion assay. The presence of zinc ions changes the course of the above reaction, and illustrates the use of zinc as a "blocking function".

Studies on Bovine Insulin with Methyl Vinyl Ketone: Isolation of Crystalline Al-3-Oxobutyl Derivative of Bovine Insulin