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Bacterial proteases are considered virulence factors and it is presumed that by abrogating their activity, host endogenous protease inhibitors play a role in host defense against invading pathogens. Here we present data showing that  Staphylococcus aureus  cysteine proteases (staphopains) are efficiently inhibited by Squamous Cell Carcinoma Antigen 1 (SCCA1), an epithelial-derived serpin. The high association rate constant ( k  ass ) for inhibitory complex formation (1.9×10 4  m /s and 5.8×10 4   m /s for staphopain A and staphopain B interaction with SCCA1, respectively), strongly suggests that SCCA1 can regulate staphopain activity  in vivo  at epithelial surfaces infected/colonized by  S. aureus . The mechanism of staphopain inhibition by SCCA1 is apparently the same for serpin interaction with target serine proteases whereby the formation of a covalent complex result in cleavage of the inhibitory reactive site peptide bond and associated release of the C-terminal serpin fragment. Interestingly, the SCCA1 reactive site closely resembles a motif in the reactive site loop of native  S. aureus -derived inhibitors of the staphopains (staphostatins). Given that  S. aureus  is a major pathogen of epithelial surfaces, we suggest that SCCA1 functions to temper the virulence of this bacterium by inhibiting the staphopains.

Inhibition of Staphylococcus aureus cysteine proteases by human serpin potentially limits staphylococcal virulence