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Experimental preparations based on a DNA vaccine encoding the ESAT-6 antigen of  Mycobacterium tuberculosis  have been obtained (KpONE6) and studied for immunogenic effects in the murine model. The core of the preparation contains DNA of the recombinant plasmid pONE6 encapsulated within a spermidine-polyglucin conjugate, thereby protecting the DNA vaccine from degradation. KpONE6 induces a proliferative T-cell immune response in mice upon intramuscular immunization.

Construction of an encapsulated ESAT-6-based anti-TB DNA vaccine and evaluation of its immunogenic properties