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Recently, a new member of the human SR (Ser/Arg-rich) superfamily of pre-mRNA splicing factors, SRA1 (SR-A1), has been discovered and cloned by members of our group, the gene for which was found to be overexpessed in a series of human tumors. In the present study, we investigated the significance of alterations at the mRNA expression levels of the  SRA1  gene after treatment of HL-60 human promyelocytic leukemia cells with the anticancer drugs cisplatin and bleomycin. The kinetics of apoptosis and cell toxicity were investigated by DNA laddering and the MTT and trypan blue assays, respectively. Total RNA was extracted and cDNA was prepared by reverse transcription. The splicing-related genes  SRA1  and  SC35 , as well as the apoptosis-related gene  BCL2  ( Bcl-2 ), were amplified by PCR using gene-specific primers. The results showed that mRNA levels of  SRA1  were up-regulated upon treatment with the antibiotic bleomycin, whereas they were down-regulated by treatment of HL-60 human promyelocytic leukemia cells with cisplatin. Our results support the hypothesis that mRNA expression analysis of  SRA1  may serve as a new prospective molecular marker, playing an important role in chemotherapy outcome in human leukemia.

biological chemistry

Effect of bleomycin and cisplatin on the expression profile of  SRA1 , a novel member of pre-mRNA splicing factors, in HL-60 human promyelocytic leukemia cells