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Calodium hepaticum  is a parasitic nematode found primarily in rodents but known to infect numerous other mammal species, and causing in humans the hepatic calodiasis. Herein, we present the first finding of  C. hepaticum  in  Rattus norvegicus  in Poland. In this study, we have used the combined pepsin-HCl digestion and molecular techniques to detect  C. hepaticum  infection in the liver. The results confirm the usefulness of molecular approaches for investigating the biology and epidemiology of  C. hepaticum . Additionally in this study, the parts of the small subunit rRNA (18S rRNA) gene of  Aonchotheca annulosa  from bank vole,  Aonchotheca erinacei  and  Eucoleus  sp. from hedgehog were amplified, yielding the first 18S rRNA gene sequences of these Capillariinae nematodes.

The molecular identification of Calodium hepaticum in the wild brown rat ( Rattus norvegicus ) in Poland