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Two species of parasitic nematodes collected from the intestine of Brook’s House Gecko,  Hemidactylus brooki  and American cockroach,  Periplaneta americana  L. The parasites were identified as  Thelandros scleratus  and  Thelastoma icemi  by PCR amplification method. Subsequently, sequence analysis of mt  cox 1 (504 and 540 bp) for  T. scleratus  and  T. icemi  respectively revealed that these sequences showed maximum similarity of 90% (in case of  T. scleratus ), 77% (in case of  T. icemi ), to nematode sequences available on GenBank. To our knowledge, no  cox 1 sequence is available for both the species of family Pharyngodonidae and Thelastomatidae. This study represents the first mitochondrial DNA characterization of both species.

Molecular identification of Thelandros scleratus and Thelastoma icemi (Nematoda: Oxyruida) using mitochondrial cox 1 sequences