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Parasitic nematodes of  Oesophagostomum spp ., commonly known as ‘nodular worms’ are one of the most widely distributed and prevalent emerging zoonotic nematodes. However, little is known about the prevalence and gene characteristics of those parasites in Tibetan pigs. Therefore, a study was carried out to investigate the prevalence, isolation and identification of  Oesophagostomum spp  from Tibetan pigs by genetic markers of  nad1 , cox3  and  ITS1  for the first time. The results revealed that the infection rate of  O. dentatum  and  O. quadrispinulatum  by genetic markers of nad1 was 81.13%; 35 (66.04%); the  O. dentatum  and  O. quadrispinulatum  by genetic markers  cox3  was 66.04%, and  O. dentatum  and  O. stephanostomum  by genetic markers  ITS1  was found to be 77.36%. Interestingly, the  O. stephanostomum  specie was identified and isolated from 90.48% stomach and 69.23% colon samples by genetic markers of  ITS1 . The present study, for the first time has described the presence and genetic characterization of  Oesophagostomun spp  of  O. dentatum ,  O. quadrispinulatum  and especially  O. stephanostomum  in Tibetan pigs from the high and remote Tibetan plateau. A public concern should be raised in terms of economical losses and severe public health problem.

Prevalence of three Oesophagostomum spp . from Tibetan Pigs analyzed by Genetic Markers of nad1 , cox3 and ITS1